Title Human retroviruses : methods and protocols / edited by Elisa Vicenzi and Guido Poli, Division of Immunology, Transplant, and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy

Published New York : Humana Press, [2014] ©2014

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Description 1 online resource (xviii, 345 pages) : illustrations (some color) Series Methods in Molecular Biology, 1940-6029 ; Volume No. 1087 Methods in molecular biology (Clifton, N.J.) ; v. 1087. 1064-3745 Contents Part I. Mapping the HIV Life Cycle -- Virion Attachment and Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand-Binding Assay / Claudia Cicala and James Arthos -- CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5[alpha] / Gongpu Zhao and Peijun Zhang -- The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating / Yang Yang, Jeremy Luban, and Felipe Diaz-Griffero -- The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells / Amy E. Hulme and Thomas J. Hope -- Imaging HIV-1 Nuclear Pre-integration Complexes / Anna Cereseto and Mauro Giacca -- HIV-1 Reverse Transcription / Andrea Cimarelli and Jean-Luc Darlix -- RNase H: Specificity, Mechanisms of Action, and Antiviral Target / Karin Moelling, Felix Broecker, and John E. Kerrigan -- HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat / Laurence Colin, Eric Verdin, and Carine Van Lint -- HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export / Alan Cochrane -- HIV-1 Accessory Proteins: Nef / Anke Heigele, Daniel Sauter, Jan Münch, and Frank Kirchhoff -- HIV-1 Accessory Proteins: VpR / Richard Y. Zhao and Michael I. Bukrinsky -- HIV-1 Accessory Proteins: Vpu and Vif / Amy Andrew and Klaus Strebel -- SIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells / Gregory Berger and Andrea Cimarelli -- Imaging of HIV Assembly and Release / Barbara Müller and Jacomine Krijnse-Locker Part II. Isolation, Co-Receptor Use, and Cell Tropism of HIV-1 -- HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells / Stefania Dispinseri, Elisa Saba, Elisa Vicenzi, Neeltje A. Kootstra, Hanneke Schuitemaker, and Gabriella Scarlatti -- Determination of HIV-1 Co-receptor Usage / Mariangela Cavarelli and Gabriella Scarlatti -- The Macrophage and HIV: Basic Concepts and Methodologies / Suzanne Gartner -- HIV Infection of Dendritic Cells / Najla Nasr, Andrew Harman, Stuart Turville, and Anthony L. Cunningham -- Histocultures (Tissue Explants) in Human Retrovirology / Anush Arakelyan, Wendy Fitzgerald, Jean-Charles Grivel, Christophe Vanpouille, and Leonid Margolis Part III. In Vivo Quantification of HIV-1 -- Single-Copy Quantification of HIV-1 in Clinical Samples / Ann Wiegand and Frank Maldarelli -- Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells / Christine Rouzioux, Adeline Mélard, and Véronique Avéttand-Fénoël Part IV. Biotechnological Aspects of HIV-1 -- HIV-1-Based Lentiviral Vectors / Ying Poi Liu and Ben Berkhout -- Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone / Zachary A. Klase, Laurent Houzet, and Kuan-Teh Jeang Part V. HTLVS -- Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques / Greta Forlani, Roberto S. Accolla, and Giovanna Tosi -- Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR / Claudio Casoli, Elisabetta Pilotti, and Umberto Bertazzoni -- Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real-Time RT-PCR (qRT-PCR) / Ilaria Cavallari, Francesca Rende, and Vincenzo Ciminale Summary "Human Retroviruses: Methods and Protocols collects key experimental protocols that have provided the basis of the major discoveries of the field. Split into five sections, this detailed volume covers mapping of the HIV life cycle, isolation, co-receptor use, and cell tropism of HIV-1, in vivo quantification of HIV-1, biological aspects of HIV-1, as well as HTLVs. Some articles explore assay and function of accessory genes, largely involving the interface between retroviral and host factors, the extracellular role of Tat and Tax, resembling the function of cytokines, and the biotechnological exploitation of HIV as lentiviral vector to carry foreign genes with therapeutic value. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Human Retroviruses: Methods and Protocols provides state-of-art methodological protocols from world leaders in human retrovirology, essential for any lab working this vital field."--Publisher's description Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (Springer Protocols, viewed January 30, 2014) Subject Retroviruses -- Laboratory manuals HIV (Viruses) -- Laboratory manuals HTLV (Viruses) -- Laboratory manuals RNA viruses. Oncogenic viruses. Lentiviruses. Viruses. HIV (Viruses) Lentiviruses, Primate RNA Viruses Oncogenic Viruses Publication Formats Vertebrate Viruses Lentivirus Viruses Publication Characteristics Organisms HIV Retroviridae Laboratory Manuals Viruses RNA viruses Oncogenic viruses Lentiviruses HIV (Viruses) HTLV (Viruses) Retroviruses Genre/Form Laboratory manuals Form Electronic book Author Vicenzi, Elisa, editor Poli, Guido, editor LC no. 2013949423 ISBN 9781627036702 1627036709 1627036695 9781627036696

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