| Description |
1 online resource (xiii, 341 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; v. 1286 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1286. 1064-3745
|
| Contents |
Affinity chromatography : a historical perspective / David S. Hage and Ryan Matsuda -- Protein purification by aminosquarylium cyanine dye-affinity chromatography / Vania C. Graca [and others] -- One-step purification of phosphinothricin acetyltransferase using reactive dye-affinity chromatography / Cunxi Wang [and others] -- Antibody purification from human plasma by metal-chelated affinity membranes. / Handan Yavuz [and others] -- Specific recognition of supercoiled plasmid DNA by affinity chromatography using a synthetic aromatic ligand / Catarina Caramelo-Nunes and Candida T. Tomaz -- Cell affinity separations on microfluidic devices / Yan Gao, Wenjie Li, Ye Zhang, and Dimitri Pappas -- Aptamer-modified magnetic beads in affinity separation of proteins / Guohong Zhu and Johanna-Gabriela Walter -- Strep-tag system for one-step affinity purification of proteins from mammalian cell culture / Thomas Schmidt and Arne Skerra -- Robotic high-throughput purification of affinity-tagged recombinant proteins / Simone C. Wiesler and Robert O.J. Weinzierl -- Macroporous silica particles derivatized for enhanced lectin affinity enrichment of glycoproteins / Benjamin F. Mann -- Immobilized magnetic beads-based multi-target affinity selection coupled with HPLC-MS for screening active compounds from traditional chinese medicine and natural products / Yaqi Chen, Zhui Chen, and Yi Wang -- Mixed-bed affinity chromatography : principles and methods / Egisto Boschetti and Pier Giorgio Righetti -- Preparation and characterization of fluorophenylboronic acid-functionalized affinity monolithic columns for the selective enrichment of cis-diol-containing biomolecules / Qianjin Li and Zhen Liu -- Introduction to macroporous cryogels / Senta Reichelt -- Cryogels with affinity ligands as tools in protein purification / Solmaz Hajizadeh and Bo Mattiasson -- Direct capture of His6-tagged proteins using megaporous cryogels developed for metal-ion affinity chromatography / Naveen Kumar Singh [and others] -- Ni(II) chelated IDA functionalized poly(HEMA-GMA) cryogels for urease adsorption / Murat Uygun -- Novel chromatographic media : histidine-containing composite cryogels for HIgG separation from human serum / Gozde Baydemir and Mehmet Odabasi -- Molecularly imprinted cryogels for human serum albumin depletion / Muge Andac [and others] -- Interpenetrating polymer network composite cryogels with tailored porous morphology and sorption properties / Ecaterina Stela Dragan and Maria Valentina Dinu -- Analysis of drug-protein interactions by high-performance affinity chromatography : interactions of sulfonylurea drugs with normal and glycated human serum albumin / Ryan Matsuda [and others] -- Accurate protein-peptide titration experiments by nuclear magnetic resonance using low-volume samples / Christian Kohler [and others] -- Characterization of the binding strengths between boronic acids and cis-diol-containing biomolecules by affinity capillary electrophoresis / Chenchen Lu and Zhen Liu -- Determination of the kinetic rate constant of cyclodextrin supramolecular systems by high-performance affinity chromatography / Jiwen Zhang [and others] -- Molecular modeling of the affinity chromatography of monoclonal antibodies / Matteo Paloni and Carlo Cavallotti |
| Summary |
The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols, OCLCbrA0 molecular modelling, OCLCbrA0 and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. OCLCbrA0 OCLCbrA0 Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is OCLCbrA0 designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity |
| Notes |
Revised edition of: Affinity chromatography / edited by Michael Zachariou, c2008 |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Affinity chromatography -- Laboratory manuals
|
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Chromatography, Affinity -- methods
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Affinity chromatography
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
|
| Author |
Reichelt, Senta, editor
|
| ISBN |
9781493924479 |
|
1493924478 |
|
149392446X |
|
9781493924462 |
|
