Title T-cell motility : methods and protocols / edited by Navin Kumar Verma

Published New York : Humana Press : Springer, [2019]

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Description 1 online resource (x, 158 pages) : illustrations (chiefly color) Series Methods in molecular biology, 1940-6029 ; volume 1930 Methods in molecular biology (Clifton, N.J.) ; v. 1930. 1064-3745 Contents Introduction to LFA-1/ICAM-1 interactions in T-cell motility / Navin Kumar Verma and Dermot Kelleher -- Isolation of human peripheral blood T-lymphocytes / Atish Kizhakeyil, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, and Navin Kumar Verma -- Laboratory model to study T-cell motility / Navin Kumar Verma, Shyam Mohan Palapetta, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Atish Kizhakeyil, and Dermot Kelleher -- Quantification of T-cell migratory phenotypes using high-content analysis / Aik Seng Ng, Seow Theng Ong, Dermot Kelleher, and Navin Kumar Verma -- Live cell imaging and analysis to capture T-cell motility in real-time / Seow Theng Ong and Navin Kumar Verma -- Three-dimensional structured illumination microscopy (3D-SIM) to dissect signaling cross-talks in motile T-cells / Seow Theng Ong, Graham D. Wright, and Navin Kumar Verma -- Real-time impedance-based detection of LFA-1-stimulated T-cell transwell chemotaxis / Praseetha Prasannan and Navin Kumar Verma -- Quantitative real-time PCR for Evaluating Transcriptional Changes in T-Lymphocytes / Atish Kizhakeyil and Navin Kumar Verma -- GapmeR-mediated gene silencing in motile T-cells / Mobashar Hussain Urf Turabe Fazil, Seow Theng Ong, Madhavi Latha Somaraju Chalasani, Atish Kizhakeyil, and Navin Kumar Verma -- Live imaging of resident T-cell migration in human lymphoid tissue slices using confocal microscopy / Emmanuel Donnadieu, Yvonne Michel, and Martin-Leo Hansmann -- Phosphoprotein enrichment for protein analysis in motile T-lymphocytes / Praseetha Prasannan, Rathi Saravanan, and Navin Kumar Verma -- Immunometabolomic phenotyping of motile T-cells / Madhavi Latha Somaraju Chalasani, Gaelin Looi Wen Dong, and Navin Kumar Verma -- Profiling activity of cellular kinases in migrating T-cells / Chandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy, Navin Kumar Verma, and WimVanden Berghe -- Biotinylation assay to determine LFA-1 recycling in motile T-lymphocytes / Malin Samuelsson and Lena M. Svensson -- Protocol to study T-cell signaling in an immune synapse by microscopy / Mobashar Hussain Urf Turabe Fazil, Pankaj Kumar, and Navin Kumar Verma -- Enzyme-linked immunosorbent assay for T-cell dependent immunogenicity assessment of therapeutic peptides / Madhavi Latha Somaraju Chalasani, Rajamani Lakshminarayanan, and Navin Kumar Verma -- Modified intravital microscopy to assess vascular health and T-cell motility / Geoffrey W. Payne, Kevin Mitchell, and Stephanie L. Sellers -- Computational analysis of protein-protein interactions in motile T-cells / Sunil Kumar, Mobashar Hussain Urf Turabe Fazil, Khurshid Ahmad, Minaketan Tripathy, Jagath Chandana Rajapakse, and Navin Kumar Verma Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerLink, viewed January 7, 2019) Subject T cells -- Laboratory manuals Cells -- Motility -- Laboratory manuals T-Lymphocytes -- physiology T-Lymphocytes -- immunology Cell Movement -- physiology Cells -- Motility T cells Genre/Form Electronic books Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Verma, Navin Kumar, editor ISBN 9781493990368 1493990365

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