| Description |
1 online resource (xvii, 502 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 |
|
Methods in molecular biology (Clifton, N.J.), 1940-6029
|
| Contents |
Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness -- The MTT Assay: A Method for Error Minimization and Interpretation in Measuring Cytotoxicity and Estimating Cell Viability -- Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditions -- Network Reconstruction as a Novel High-Level Marker of Functional Neuronal Viability -- Assaying Mitochondrial Function by Multi-Parametric Flow Cytometry -- High-Efficiency Single Cell Electrical Impedance Spectroscopy -- Cell Viability Multiplexing: Quantification of Cellular Viability by Barcode Flow Cytometry and Computational Analysis -- Average Rheological Quantities of Cells in Monolayers -- Assaying Spontaneous Network Activity and Cellular Viability Using Multi-Well Microelectrode Arrays -- Quantitative Live-Cell Ca2+ Imaging During Isotropic Cell Stretch -- Assessment of Cell Viability in Electrically Excitable Muscle Cells through Intact Twitch Stimulation -- Evaluating Cellular Viability by iTRAQ Proteomic Profiling -- Quantifying pH in Malaria Using pHluorin and Flow Cytometry -- Cell Membrane State, Permeability, and Elasticity Assessment for Single Cells and Cell Ensembles -- Neutral Red Uptake Assay to Assess Cytotoxicity In Vitro -- Digital Holographic Microscopy to Assess Cell Behavior -- Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells -- Optimization of Cell Viability Assays for Drug Sensitivity Screens -- Cellasys #8: A Microphysiometric Assay for Real-Time Cell Analysis within 24 Hours -- Live Cell Adhesion, Migration, and Invasion Assays -- Cell Viability and Immunogenic Function of T Cells Loaded with Nanoparticles for Spatial Guidance in Magnetic Fields -- Transwell In Vitro Cell Migration and Invasion Assays -- Live Cell Calcium Imaging of Non-Adherent Cells -- FUCCI Reporter Gene-Based Cell Cycle Analysis -- Cell Viability Assays for 3D Cellular Constructs -- Toxicological Analysis by Assessment of Vascularization and Cell Viability Using the Chicken's Chorioallantoic Membrane (CAM Assay) -- Assessment of Tissue Viability by Functional Imaging of Membrane Potential -- Multiplexed Viability Assays for High-Throughput Screening of Spheroids of Multiple Sizes -- Tracking Gut Motility in Organ and Cultures -- Optimized Method of 3D Scaffold Seeding, Cell Cultivation, and Monitoring Cell Status for Bone Tissue Engineering |
| Summary |
This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book's contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the "wellbeing" of cells in various environments |
| Notes |
Includes index |
|
Online resource; title from PDF title page (SpringerLink, viewed May 10, 2023) |
| Subject |
Cytology -- Laboratory manuals
|
|
Biological assay -- Laboratory manuals
|
|
Biological assay
|
|
Cytology
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Friedrich, Oliver, editor.
|
|
Gilbert, Daniel F., editor.
|
| ISBN |
9781071630525 |
|
1071630520 |
|
