Title Cell tracking : methods and protocols / edited by Matthew T. Basel and Stefan H. Bossmann

Published New York : Humana Press, 2020

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Description 1 online resource (x, 192 pages) : illustrations Series Methods in molecular biology, 1940-6029 ; volume 2126 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 2126. 1064-3745 Springer protocols (Series) 1949-2448 Contents Using Bioengineered Bioluminescence to Track Stem Cell Transplantation In Vivo -- Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations -- In vivo Epithelial Metabolic Imaging using a Topical Fluorescent Glucose Analog -- Lipophilic Near Infrared Dyes for in vivo Fluorescent Cell Tracking -- Energy-transfer Systems for in vivo Tracking -- Using Bioengineered Fluorescence for Selective in vivo and ex vivo Tracking of Intestinal Organoids Derived from Human Pluripotent Stem Cells -- Using iRFP Genetic Labeling Technology to Track Tumorogenesis of Transplanted CRISPR/Cas9-edited iPSC in Skeletal Muscle -- Upconversion Fluorescent Nanoprobe for Highly-sensitive in vivo Cell Tracking -- Magnetic Resonance Imaging of Single Cells -- MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles -- Protease-activated Sensors for In Vivo Imaging of Cell Populations -- Antibody Targeted Magnetic Nanoparticles to Track Immune Cells in Vivo -- Stem Cell Tracking with Nanoparticle-Based Ultrasound Contrast Agents -- Gold Nanoparticles as Computer Tomography Marker for Stem Cell Tracking -- Non-Invasive Tracking of Implanted Cells: Superparamagnetic Iron Oxide Nanoparticles as a Long-Term, Multi-Modal Imaging Label -- Comparison of the Efficacy and Sensitivity of Alternative PET Reporter Gene/PET Reporter Probe Systems that Minimizes Biological Variables Summary This volume details methods used to track cells in the body and will serve as a reference for preclinical and clinical researchers in the fields of medicine and biomedical science. Chapters guide readers through protocols on bioluminescence imaging, fluorescence imaging, magnetic resonance imaging (MRI), ultrasound, computed tomography, and positron emission spectroscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell Tracking: Methods and Protocols aims to ensure successful results in the further study of this vital field Bibliography Includes bibliographical references and index Subject Cells -- Imaging -- Laboratory manuals Cells -- Motility -- Laboratory manuals Cell Tracking -- methods Cell Movement Cellular biology (cytology) Science -- Life Sciences -- Biology -- Molecular Biology. Cells -- Motility Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Basel, Matthew T., editor Bossmann, Stefan H., editor ISBN 9781071603642 1071603647 9781071603635 1071603639

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