Title Protein-ligand interactions : methods and applications / edited by Mark A. Williams, Tina Daviter

Edition 2nd ed Published New York : Humana Press, ©2013

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Description 1 online resource (x, 530 pages) : illustrations Series Methods in molecular biology, 1940-6029 ; v. 1008 Methods in molecular biology (Clifton, N.J.) ; volume 1008. 1064-3745 Contents Protein-ligand interactions : fundamentals / Mark A. Williams -- Protein sample characterization / Tina Daviter and Remi Fronzes -- Measurement of protein-ligand complex formation / Peter N. Lowe, Cara K. Vaughan, and Tina Daviter -- Isothermal titration calorimetry for studying protein-ligand interactions / Luminita Damian -- Rapid mixing kinetic techniques / Stephen R. Martin and Maria J. Schilstra -- Protein-ligand interactions using SPR systems / Asa Frostell, Lena Vinterback, and Hans Sjobom -- Fluorescence techniques in analysis of protein-ligand interactions / Gabor Mocz and Justin A. Ross -- Circular and linear dichroism spectroscopy for the study of protein-ligand interactions / Tina Daviter, Nikola Chmel, and Alison Rodger -- Analyzing protein-ligand interactions by dynamic NMR spectroscopy / Anthony Mittermaier and Erick Meneses -- Studying metal ion-protein interactions : electronic absorption, circular dichroism, and electron paramagnetic resonance / Liliana Quintanar and Lina Rivillas-Acevedo -- Monitoring protein-ligand interactions by time-resolved FTIR difference spectroscopy / Carsten Kotting and Klaus Gerwert -- Biophysical methods in drug discovery from small molecule to pharmaceutical / Geoffrey Holdgate [and others] -- Biophysical screening for the discovery of small-molecule ligands / Alessio Ciulli -- Screening protein-small molecule interactions by NMR / Ben Davis -- Model membrane systems / Heiko Keller, Remigiusz Worch, and Petra Schwille -- Quantitative fluorescence co-localization to study protein-receptor complexes / Shanica N. Pompey, Peter Michaely, and Katherine Luby-Phelps -- Studying protein-ligand interactions using X-ray crystallography / Andrew P. Turnbull and Paul Emsley -- Molecular fields in ligand discovery / Paul J. Gane and A.W. Edith Chan -- Structure-based virtual screening for novel ligands / William R. Pitt [and others] Summary Proteins are the cell's workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions - whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology Analysis Life sciences Biochemistry RNA-ligand interactions Protein Science Protein-Ligand Interactions Bibliography Includes bibliographical references and index Notes English Subject Proteins -- Laboratory manuals Ligands -- Laboratory manuals Protein binding -- Laboratory manuals Ligand binding (Biochemistry) -- Laboratory manuals Ligands. Carrier proteins. Ligands Proteins -- chemistry Chemistry, Analytical -- methods Carrier Proteins Carrier proteins Ligand binding (Biochemistry) Ligands Protein binding Proteins Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Williams, Mark A. Daviter, Tina. ISBN 9781627033985 162703398X 1627033971 9781627033978

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