Title Nuclear G-protein coupled receptors : methods and protocols / edited by Bruce G. Allen, Terence E. Hébert

Published New York, NY : Humana Press, [2015] ©2015

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Description 1 online resource (xv, 215 pages) : illustrations (some color) Series Methods in Molecular Biology, 1940-6029 ; 1234 Methods in molecular biology (Clifton, N.J.) ; v. 1234. 1064-3745 Contents Studies of intracellular angiotensin II / Richard N. Re and Julia L. Cook -- Single-cell microinjection coupled to confocal microscopy to characterize nuclear membrane receptors in freshly isolated cardiomyocytes / Clémence Merlen and Jonathan Ledoux -- Design and application of light-activated probes for cellular signaling / David Chatenet, Steve Bourgault, and Alain Fournier -- Using caged ligands to study intracrine endothelin signaling in intact cardiac myocytes / Clémence Merlen, Louis R. Villeneuve, and Bruce G. Allen -- Quantification of catecholamine uptake in adult cardiac myocytes / Erika F. Dahl, Casey D. Wright, and Timothy D. O'Connell -- Characterization of the interaction between the prostaglandin D2 DP1 receptor and the intracellular L-Prostaglandin D synthase / Chantal Binda and Jean-Luc Parent -- Isolation and study of cardiac nuclei from canine myocardium and adult ventricular myocytes / Artavazd Tadevosyan, Bruce G. Allen, and Stanley Nattel -- High resolution imaging and function of nuclear G protein-coupled receptors (GPCRs) / Vikrant K. Bhosle [and others] -- Biochemical fractionation of membrane receptors in the nucleus / Ying-Nai Wang ... [et at.] -- Functional G protein-coupled Receptors on nuclei from brain and primary cultured neurons / Yuh-Jiin I. Jong and Karen L. O'Malley -- Automated microscopy of cardiac myocyte hypertrophy : a case study on the role of intracellular [alpha]-adrenergic receptors / Karen A. Ryall and Jeffrey J. Saucerman -- Measuring intranuclear and nuclear envelope [Ca2+] vs. cytosolic [Ca2+] / Senka Ljubojević and Donald M. Bers -- Assessing GPCR and G protein signaling to the nucleus in live cells using fluorescent biosensors / Julie Bossuyt and Donald M. Bers -- Tandem affinity purification to identify cytosolic and nuclear G [beta-gamma]-interacting proteins / Rhiannon Campden [and others] -- Examining the effects of nuclear GPCRs on gene expression using isolated nuclei / George Vaniotis [and others] -- Trafficking and function of GPCRs in the endosomal compartment / Davide Calebiro [and others] Summary Nuclear G-Protein Coupled Receptors: Methods and Protocols is a compilation of a number of conceptual and methodological aspects important for the validation and characterization of intacrine signaling systems. To date, the best-characterized intracrine signaling system is that of angiotensin II (Ang II), covered in depth in various chapters. Methodology to study the subcellular localization and function of GPCRs and other signaling systems is provided, as well as numerous chapters focusing on methods designed to understand signaling mediated by nuclear and other internal GPCRs. Methods are also described to study the formation of second messengers such as cAMP and to study the trafficking of receptors from the cell surface. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Nuclear G-Protein Coupled Receptors: Methods and Protocols seeks to serve both professionals and novices with state-of-the-art approaches to characterize what is becoming a common theme in cellular signaling Analysis eiwitten proteins Proteins and Enzymes Eiwitten en enzymen Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerLink, viewed October 28, 2014) Subject G proteins -- Receptors -- Laboratory manuals Carrier proteins. Genetic regulation. Receptors, G-Protein-Coupled Carrier Proteins Cell Nucleus Structures Gene Expression Regulation Signal Transduction -- physiology Proteins. Science -- Life Sciences -- Biochemistry. Genetic regulation Carrier proteins G proteins -- Receptors Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Allen, Bruce G., editor. Hébert, Terence E., editor. ISBN 9781493917556 1493917552 1493917544 9781493917549

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