Title Plant cell culture protocols / edited by Victor M. Loyola-Vargas and Felipe Vázquez-Flota

Edition 2nd ed Published Totowa, N.J. : Humana Press, ©2006

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Description 1 online resource (xv, 393 pages) : illustrations (some color) Series Methods in molecular biology, 1064-3745 ; 318 Methods in molecular biology (Clifton, N.J.) ; v. 318. Contents An introduction to plant cell culture: Back to the future / V.M. Loyola-Vargas and F. Vazquez-Flota -- History of plant tissue culture / T.A. Thorpe -- Pathogen and biological contamination management: the road ahead / A.C. Cassells and B.M. Doyle -- Growth measurements: estimation of cell division and cell expansion / G. Godoy-Hernandez and F.A. Vazquez-Flota -- Callus and suspension culture induction, maintenance, and characterization / A. Robledo-Paz [and others] -- Measurement of cell viability in in vitro cultures / L.A. Castro-Concha, R.M. Escobedo and M.L. Miranda-Ham -- Cryopreservation of embryogenic cell suspensions by encapsulation-vitrification / Q. Wang and A. Perl -- Somatic embryogenesis in Picea suspension cultures / C. Stasolla -- Indirect somatic embryogenesis in cassava for genetic modification purposes / K. Raemakers [and others] -- Direct somatic embryogenesis in Coffea canephora / F. Quiroz-Figueroa [and others] -- A new temporary immersion bioreactor system for micropropagation / M.L. Robert [and others] -- Protocol to achieve photoautotrophic coconut plants cultured in vitro with improved performance ex vitro / G. Fuentes [and others] -- Use of statistics in plant biotechnology / M.E. Compton -- An efficient method for the micropropagation of Agave species / M.L. Robert [and others] -- Micropropagation of endangered plant species / Z. Liao [and others] -- Clonal propagation of softwoods / T.A. Thorpe, I.S. Harry and E.C. Yeung -- Isolation, culture, and plant regeneration from leaf protoplasts of Passiflora / M.R. Davey [and others] -- Isolation, culture, and plant regeneration from Echinacea purpurea protoplasts / Z.G. Pan [and others] -- Production of cybrids in Brassicaceae species / M. Vasylenko [and others] -- Guard cell protoplasts: isolation, culture, and regeneration of plants / G. Tallman -- Production of interspecific hybrid plants in Primula / J. Kato and M. Mii -- Agrobacterium-mediated transformation of Petunia leaf discs / I.M. van der Meer -- Transformation of wheat via particle bombardment / I.K. Vasil and V. Vasil -- Chloroplast transformation / X.M. Lu, W.B. Yin and Z.M. Hu -- The biochemical basis for the resistance to aluminum and their potential as selection markers / S.B. De los and T. Hernandez-Sotomayor -- Transformation of maize via Agrobacterium tumefaciens using a binary co-integrate vector system / Z.Y. Zhao and J. Ranch -- Capsaicin accumulation in Capsicum spp. suspension cultures / N. Ochoa-Alejo -- Isolation and purification of ribosome-inactivating proteins / S.W. Park [and others] -- Catharanthus roseus shoot cultures for the production of monoterpenoid indole alkaloids / E. Hernandez-Dominguez [and others] -- Methods for regeneration and transformation in Eschscholzia californica: A model plant to investigate alkaloid biosynthesis / B.P. MacLeod and P.J. Facchini -- The components of the culture media / V.M. Loyola-Vargas -- Plant biotechnology and tissue culture resources on the internet / V.M. Loyola-Vargas Summary Following in the footsteps of its much-acclaimed first edition, Plant Cell Culture Protocols, Second Edition has been expanded and revised to include the most up-to-date and frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Two new appendices have also been added to explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, as well as to list more than 100 very useful internet sites. Comprehensive and highly practical, Plant Cell Culture Protocols, Second Edition offers today's plant biotechnologists indispensable state-of-the-art techniques for carrying out plant research, for developing new transgenic plants, and for today's many and diverse commercial applications Bibliography Includes bibliographical references Notes English Print version record Subject Plant cell culture -- Laboratory manuals Plant tissue culture -- Laboratory manuals Cell culture. Cultures (Biology) Cells. Medical laboratory technology. Anatomy. Plant Cells Cell Culture Techniques Cells, Cultured Culture Techniques Cells Clinical Laboratory Techniques Anatomy Investigative Techniques Analytical, Diagnostic and Therapeutic Techniques and Equipment anatomy. SCIENCE -- Life Sciences -- Cell Biology. Medical laboratory technology Cultures (Biology) Cells Cell culture Anatomy Plant cell culture Plant tissue culture Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Loyola-Vargas, Victor M Vázquez-Flota, Felipe LC no. 2005046025 ISBN 1588295478 9781588295477 9781592599592 1592599591 1280358807 9781280358807 9786610358809 661035880X

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