| Description |
1 online resource (xiv, 509 pages) : illustrations (some color) |
| Series |
Methods in Molecular Biology, 1940-6029 ; 1312 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1312. 1064-3745
|
| Contents |
Early days of blotting / Edwin Southern -- Origins of protein blotting / Harry Towbin -- Western blotting : remembrance of things past / W. Neal Burnette -- Simian virus 40 and protein transfer / Jaime Renart -- Western blotting : an introduction / Biji T. Kurien and R. Hal Scofield -- From little helpers to automation / Biji T. Kurien and Harry Towbin -- Spectrophotometric methods to determine protein concentration / J.P. Dean Goldring -- Solubilization of proteins : the importance of lysis buffer choice / Mandy Peach [and three others] -- Simultaneous immunoblotting analysis with activity gel electrophoresis and 2-D gel electrophoresis / Der-Yen Lee and Geen-Dong Chang -- Diffusion blotting : a rapid and simple method for production of multiple blots from a single gel / Ingrid Olsen and Harald G. Wiker -- Multiple immunoblots by passive diffusion of proteins from a single SDS-PAGE gel / Biji T. Kurien and R. Hal Scofield -- Slice blotting / Graeme Lowe -- Localizing proteins by tissue printing / Rafael F. Pont-Lezica -- Dot-immunobinding assay (Dot-Iba) / Sumi Surendran, Annamma Mathai, and Vishnampet Venkataraman Radhakrishnan -- Analysis of antibody clonotype by affinity immunoblotting / Biji T. Kurien and R. Hal Scofield -- Glycosaminoglycan blotting and detection after electrophoresis separation / Nicola Volpi and Francesca Maccari -- Well-based reverse-phase protein array of formalin- fixed paraffin-embedded tissue / Joon-Yong Chung and Stephen M. Hewitt -- Quantitative computerized western blotting in detail / Dalit Talmi-Frank, Charles L. Jaffe, and Gad Baneth -- Cationic electrophoresis and eastern blotting / Engelbert Buxbaum -- Miniaturized blotting system for simultaneous detection of different autoantibodies / Ulrich Canzler [and eight others] -- Proteomic expressional profiling of a paraffin-embedded tissue by multiplex tissue immunoblotting / Joon-Yong Chungand Stephen M. Hewitt -- Post-staining electroblotting for efficient and reliable peptide blotting / Der-Yen Lee and Geen-Dong Chang -- Multistrip western blotting : a tool for comparative quantitative analysis of multiple proteins / Edita Aksamitiene, Jan B. Hoek, and Anatoly Kiyatkin -- Western blotting using PVDF membranes and its downstream applications / Setsuko Komatsu -- Blotting from phastGel to membranes by ultrasound / Joseph Kost and Aharon Azagury -- Western blotting of high and low molecular weight proteins using heat / Biji T. Kurien and R. Hal Scofield -- Membrane strip affinity purification of autoantibodies / Biji T. Kurien -- Strip immunoblotting of multiple antigenic peptides / Biji T. Kurien -- Double-blotting : a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures / Françoise Lasne -- Method for resolution and western blotting of very large proteins using agarose electrophoresis / Marion L. Greaser and Chad M. Warren -- Immunodetection of P-selectin using an antibody to its C-terminal tag / Padmaja Mehta-D'souza -- Improvements and variants of the multiple antigen blot assay-MABA : an immunoenzymatic technique for simultaneous antigen and antibody screening / Oscar Noya [and six others] -- Blotting from immobilized pH gradient gels : application to total cell lysates / Harry Towbin -- Immunoprecipitation : western blot for proteins of low abundance / Edward P. Trieu and Ira N. Targoff -- Native electrophoresis and western blot analysis (NEWeB) : methods and applications / Ioannis N. Manoussopoulos and Mina Tsagris -- Shift-western blotting : separate analysis of protein and DNA from protein-DNA complexes / Matthias Harbers -- Grid-immunoblotting / Olga Yeliosof and R. Hal Scofield -- Detection and quantification of protein-protein interactions by far-western blotting / Joshua A. Jadwin, Bruce J. Mayer, and Kazuya Machida -- Western blot analysis of adhesive interactions under fluid shear conditions : the blot rolling assay / Robert Sackstein and Robert Fuhlbrigge -- Centrifuge blotting / Jinny Paul -- Blotting of coomassie blue-stained proteins from PAGE gels to transparencies / Jinny Paul -- B-cell ELISPOT : for the identification of antigen-specific antibody-secreting cells / Hemangi B. Shah and Kristi A. Koelsch -- T cell ELISPOT : for the identification of specific cytokine-secreting T cells / Kerry M. Leehan and Kristi A. Koelsch -- Membrane microplates for one- and two-color ELISPOT and FLUOROSPOT assays / Alexander E. Kalyuzhny -- SDS-PAGE to immunoblot in one hour / Biji T. Kurien [and three others] -- Single-cell western blotting / Syed M.S. Quadri -- Protein detection by simple western analysis / Valerie M. Harris -- Western blotting using microfluidics / Pothur R. Srinivas -- Two-dimensional gel-based protein standardization verified by western blot analysis / Hisao Haniu [and three others] -- Fingerprint deposition on nitrocellulose and polyvinylidene difluoride membranes using alkaline phosphatase / Biji T. Kurien, Debashish Danda, and R. Hal Scofield -- Other notable protein blotting methods : a brief review / Biji T. Kurien and R. Hal Scofield |
| Summary |
This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Western Blotting: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field |
| Analysis |
western blotting |
|
eiwitten |
|
proteins |
|
moleculaire biologie |
|
molecular biology |
|
laboratoriummethoden |
|
laboratory methods |
|
protocollen |
|
protocols |
|
Biological Techniques |
|
Biologische technieken |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed June 12, 2015) |
| Subject |
Western immunoblotting -- Laboratory manuals
|
|
Blotting, Western -- methods
|
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Proteins.
|
|
Science -- Life Sciences -- Biochemistry.
|
|
Western immunoblotting
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Kurien, Biji T., editor.
|
|
Scofield, R. Hal, editor.
|
| ISBN |
9781493926947 |
|
1493926942 |
|
1493926934 |
|
9781493926930 |
|
