| Description |
1 online resource (414 p.) |
| Contents |
Intro -- Proteomics: A Promising Approach for Cancer Research -- Copyright -- Contents -- Contributors -- Preface -- Chapter 1 Cancer proteomics: An overview -- 1 . Introduction -- 2 . Definition and goals of proteomics -- 2.1 Need for proteomics -- 3 . Methods of protein measurement and biomarker identification -- 3.1 2D PAGE -- 3.2 Mass spectrometry -- 3.3 Protein arrays -- 3.4 Protein bioinformatics -- 4 . Biomedical applications -- 4.1 Proteomics and cancer -- 4.2 Early diagnosis of cancer -- 4.3 Determining tumor aggressiveness -- 4.4 Individualized therapy |
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5 . Mechanisms of proteomic changes in cancer -- 6 . Cancer biomarker applications -- 6.1 Cancer heterogeneity -- 6.2 Initial early detection of cancer -- 7 . Protocols for developing tumor biomarkers -- 8 . General guidelines for a good study design for biomarker discovery -- 9 . Applications of proteomics research in various cancers -- 9.1 Types of cancers -- 9.2 Biomarkers for lung cancer detection -- 9.3 Breast cancer biomarkers -- 9.4 Ovarian cancer biomarkers and implications from proteomics -- 9.5 Ovarian cancer pathogenesis -- 9.6 Etiology -- 9.7 Ovarian cancer progression |
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9.8 Targets for therapeutic means -- 10 . Can proteomics research findings in cancer be translated into clinically oriented research? -- 10.1 EGFR kinase inhibitors -- 10.2 HSP90 inhibitors -- 11 . Conclusion -- References -- Further reading -- Chapter 2 Proteomics: A groundbreaking development in cancer biology -- 1 . Introduction -- 2 . Why proteomics? -- 3 . Types of proteomics -- 3.1 Structural proteomics -- 3.2 Sequence proteomics -- 3.3 Expression proteomics -- 3.4 Functional and interaction proteomics -- 4 . Proteomics: Analytical approaches -- 5 . Conventional techniques |
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5.1 Chromatography-based techniques -- 5.2 Size exclusion chromatography -- 5.3 Ion-exchange chromatography -- 5.4 Affinity chromatography -- 5.5 ELISA (enzyme linked immunosorbent assay) -- 5.6 Western blotting -- 6 . Advanced techniques -- 6.1 Protein microarray -- 6.2 Analytical protein microarray -- 6.3 Functional protein microarray -- 6.4 Reverse-phase protein microarray -- 6.5 Edman sequencing -- 7 . Gel-based approaches -- 7.1 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) -- 7.2 Two-dimensional gel electrophoresis -- 7.3 Mass spectrometry |
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8 . Quantitative techniques -- 8.1 Stable isotopic labeling with amino acids in cell culture (SILAC) -- 8.2 Isotope-coded affinity tag (ICAT) -- 8.3 Isobaric tag for relative and absolute quantitation (iTRAQ) -- 9 . High-throughput techniques -- 9.1 X-ray crystallography -- 9.2 NMR spectroscopy -- 10 . Proteomics: A breakthrough in cancer -- 11 . Oncoproteomics -- 12 . Proteomic variations in cancer -- 13 . Necessity of proteomics in cancer -- 13.1 Early detection and diagnosis of cancer -- 14 . Heterogeneity in cancer -- 15 . Detecting tumor aggressiveness |
| Notes |
Description based upon print version of record |
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16 . Personalized cancer treatment |
| Form |
Electronic book
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| Author |
Majid, Sabhiya
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Rehman, Muneeb U
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| ISBN |
9780323950732 |
|
0323950736 |
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