| Description |
1 online resource (xi, 290 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 898 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 898. 1064-3745
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Springer protocols (Series)
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| Contents |
Metabolic engineering of Mucor circinelloides for zeaxanthin production / Marta Rodríguez-Sáiz, Juan-Luis de la Fuente, and José-Luis Barredo -- Bioengineering of oleaginous yeast Yarrowia lipolytica for lycopene production / Rick W. Ye, Pamela L. Sharpe, and Quinn Zhu -- Peroxisome targeting of lycopene pathway enzymes in Pichia pastoris / Pyung Cheon Lee -- Production, extraction, and quantification of astaxanthin by Xanthophyllomyces dendrorhous or Haematococcus pluvialis : standardized techniques / Alma Rosa Domínguez-Bocanegra -- Isolation and selection of new astaxanthin producing strains of Xanthophyllomyces dendrorhous / Diego Libkind, Martín Moliné, and Celia Tognetti -- Isolation and characterization of extrachromosomal double-stranded RNA elements in Xanthophyllomyces dendrorhous / Marcelo Baeza, María Fernández-Lobato, and Víctor Cifuentes |
| Summary |
Carotenoids are a family of yellow to orange-red terpenoid pigments synthesized by photosynthetic organisms and many bacteria and fungi. They have beneficial health effects protecting against oxidative damage, and may be responsible for the colours associated with plants and animals. In Microbial Carotenoids From Fungi: Methods and Protocols, expert researchers in the field detail many of the most up-to-date methods which are now commonly used to study carotenoids. These include methods for the manipulation and metabolic engineering of the Beta-carotene producing fungi Blakeslea trispora and Mucor circinelloides, lycopene production with the yeast Yarrowia lipolytica, peroxisome targeting of lycopene pathway enzymes in Pichia pastoris, and the manipulation of the heterobasidiomycetous yeast Xanthophyllomyces dendrorhous, which produces astaxanthin, a red xanthophyll with large importance in the aquaculture, pharmaceutical, and food industries. Additionally, the book includes a DNA assembler method for construction of zeaxanthin-producing strains of Saccharomyces cerevisiae, production of neurosporaxanthin by Neurospora and Fusarium, and production of torularhodin, torulene and b-carotene by Rhodotorula yeasts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Carotenoids From Fungi: Methods and Protocols, provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms |
| Analysis |
levenswetenschappen |
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life sciences |
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microbiologie |
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microbiology |
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Biology (General) |
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Biologie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Carotenoids.
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Fungal molecular biology.
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Fungi.
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Mushrooms.
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Biotechnology -- methods
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Carotenoids -- biosynthesis
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Fungi
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Carotenoids
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Mushrooms
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Fungi
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Carotenoids
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Fungal molecular biology
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Barredo, José-Luis
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| ISBN |
9781617799181 |
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1617799181 |
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