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Title Epitope mapping protocols / edited by Glenn E. Morris
Published Totowa, N.J. : Humana Press, ©1996

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Description 1 online resource (xiii, 416 pages) : illustrations
Series Methods in molecular biology ; v. 66
Methods in molecular biology (Clifton, N.J.) ; v. 66.
Contents Choosing a method for epitope mapping / Glenn E. Morris -- Crystallographic studies of antigen-antibody interactions / Frederick A. Saul, Pedro M. Alzari -- Epitope mapping antibody-antigen complexes by nuclear magnetic resonance spectroscopy / Irina Kustanovich, Anat Zvi -- Mapping epitopes on antigens by immunodiffusion in gel / Giuseppe A. Molinaro, William C. Eby -- A simple solid-phase competition assay with labeled antigen / Masahide Kuroki -- Epitope mapping by antibody competition: methodology and evaluation of the validity of the technique / Socrates J. Tzartos -- Epitope mapping by surface plasmon resonance in the biacore / Berit Johne -- Identifying residues in antigenic determinants by chemical modification / N. Martin Young, Raymond P. Oomen -- Epitope mapping by differential chemical modification of antigens / Hans Rudolf Bosshard -- Epitope mapping by proteolysis of antigen-antibody complexes: protein footprinting / Ronald Jemmerson -- Proteolytic fragmentation for epitope mapping / Maria R. Mazzoni, Nikolai O. Artemyev, Heidi E. Hamm -- Epitope mapping by chemical fragmentation / Glenn E. Morris -- Probing antibody-antigen interactions by mass spectrometry / Yingming Zhao, Brian T. Chait -- Epitope mapping using multipin peptide synthesis / Stuart J. Rodda, N. Joe Maeji, Gordon Tribbick -- SPOT synthesis: epitope analysis with arrays of synthetic peptides prepared on cellulose membranes / Ronald Frank, Heike Overwin -- Tea bag synthesis of positional scanning synthetic combinatorial libraries and their use for mapping antigenic determinants / Clemencia Pinilla, Jon R. Appel, Richard A. Houghten -- Epitope mapping using phage-displayed peptide libraries / Diane Dottavio -- Reiterative screening of phage-display peptide libraries with antibodies / Alexander Pereboev, Glenn E. Morris -- Homolog scanning / Lin-Fa Wang -- Epitope mapping using an oligonucleotide replacement method / Hannah Alexander -- Epitope mapping by region-specified PCR mutagenesis / Takehiko Shibata, Masayuki Ikeda -- Random fragment libraries using yeast expression plasmid / Serge Bénichou, Geneviève Inchauspé -- Epitope mapping using random fragment expression libraries in [lambda] phages / Hartmut Porzig, Kenneth D. Philipson -- Random fragment libraries displayed on filamentous phage / Lin-Fa Wang, Meng Yu -- Epitope mapping by expression of restriction enzyme or PCR fragments in bacterial plasmids / Johannes A. Lenstra, Arnoud H. Vliet -- Epitope mapping of protein antigens by expression-PCR (E-PCR) / David E. Lanar, Kevin C. Kain, Henry B. Burch -- Epitope mapping on extracellular domains of cell-surface proteins using exonuclease III / Thomas Brümmendorf, Antonius Plagge, Ullrich Treubert -- An improved method for mapping epitopes of recombinant antigens by transposon mutagenesis / Steven G. Sedgwick, Brian A. Morgan, Nguyen thi Man, Glenn E. Morris -- Incomplete polypeptides of in vitro translation for epitope localization / Lisa Djavadi-Ohaniance, Bertrand Friguet -- T-cell epitope mapping with synthetic peptides and peripheral blood mononuclear cells / Stuart J. Rodda -- Use of natural or selected mutants and variants for epitope mapping / Glenn E. Morris -- Production of panels of monoclonal antibodies by the hybridoma method / Nguyen thi Man, Glenn E. Morris -- Production of phage-display antibodies for epitope mapping / Jenny Walker, George Banting
Summary Epitope Mapping Protocols offers more than thirty protocols for antibody production and mapping-all described in detail by leading experts and illustrated by proven examples from their own work. The methods range from those that use (or can use) whole, native antigens to those that use peptides and peptide libraries and those that require antigen expressed from recombinant DNA. A method for T-cell epitopes, instructions on using "natural" variants or mutants, and techniques for producing panels of antibodies for epitope mapping are also included
Analysis epitopen
epitopes
antigenen
antigens
antilichamen
antibodies
cartografie
mapping
immunogenetica
immunogenetics
immunologie
immunology
biologische technieken
biological techniques
protocollen
protocols
Immunogenetics
Immunogenetica
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL
English
Print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
Subject Antigenic determinants -- Research -- Methodology
Antigen-antibody reactions -- Research -- Methodology
Antigenic determinants.
Antigen-antibody reactions.
Epitopes
Antigen-Antibody Reactions
Epitope Mapping -- methods
Biochemistry
Life sciences
Wetenschappelijke technieken.
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Morris, Glenn E
ISBN 9781592595525
1592595529
1280836733
9781280836732
9786610836734
6610836736