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Title Epitope mapping protocols / edited by Johan Rockberg, Johan Nilvebrant
Edition Third edition
Published New York, NY : Humana Press : Springer, [2018]


Description 1 online resource (xi, 284 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; volume 1785
Methods in molecular biology (Clifton, N.J.) ; v. 1785, 1064-3745
Contents Introduction to epitope mapping / Johan Nilvebrant and Johan Rockberg -- Epitope mapping of antibody-antigen interactions with x-ray crystallography / Moeko Toride King and Cory L. Brooks -- Mapping antibody epitopes by solution NMR spectroscopy : practical considerations / Luca Simonelli, Mattia Pedotti, Marco Bardelli, Simon Jurt, Oliver Zerbe, and Luca Varani -- Label-free cell-based assay for characterization of biomolecules and receptors / Diluka Peiris, Teodor Aastrup, Samuel Altun, Camilla Kack, Maria Gianneli, Davide Proverbio, and Lars M. Jørgensen -- Probing antibody binding sites on G protein-coupled receptors using genetically encoded photo-activatable cross-linkers / Thomas Huber and Thomas P. Sakmar -- Rapid mapping of protein binding sites and conformational epitopes by coupling yeast surface display to chemical labeling and deep sequencing / Tariq Ahmad Najar, Shruti Khare, and Raghavan Varadarajan -- Epitope mapping using yeast display and next generation sequencing / Thomas Van Blarcom, Andrea Rossi, Davide Foletti, Purnima Sundar, Steven Pitts, Zea Melton, Dilduz Telman, Lora Zhao, Wai Ling Cheung, Jan Berka, Wenwu Zhai, Pavel Strop, Jaume Pons, Arvind Rajpal, and Javier Chaparro-Riggers -- B-cell epitope mapping using a library of overlapping synthetic peptides in an enzyme-linked immunosorbent assay / Thiru Vanniasinkam, Mary D. Barton, Tongted Phumoonna Das, and Michael W. Heuzenroeder -- Array-in-well epitope mapping of phage-displayed antibodies / Urpo Lamminmaki, Gaurav Batra, and Petri Saviranta -- Epitope mapping of antibodies using bacterial cell surface display of gene fragment libraries / Anna-Luisa Volk, Francis Jingxin Hu, and Johan Rockberg -- Discontinuous epitope mapping of antibodies using bacterial cell surface display of folded domains / Anna-Luisa Volk and Johan Rockberg -- Kinetic analysis and epitope binning using surface plasmon resonance / Johan Nilvebrant -- Epitope binning of human monoclonal antibodies in classical sandwich and in-tandem orientation using the octet system based on biolayer interferometry / Lisette Bronswijk-Deddens -- Antibody specificity profiling using protein microarrays / Pedro Ramos-L opez, Jose Irizarry, Ignacio Pino, and Seth Blackshaw -- High-density antigen microarrays for the assessment of antibody selectivity and off-target binding / Ronald Sjoberg, Eni Andersson, Cecilia Hellstrom, Cecilia Mattsson, Jochen M. Schwenk, Peter Nilsson, and Burcu Ayoglu -- Multiplexed antigen bead arrays for the assessment of antibody selectivity and epitope mapping / Burcu Ayoglu, Peter Nilsson, and Jochen M. Schwenk -- Antibody biomarker discovery via in vitro evolution of peptides / John T. Ballew, Jack R. Reifert, and Patrick S. Daugherty -- Bioinformatics tools for the prediction of T-cell epitopes / Massimo Andreatta and Morten Nielsen
Summary This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements. The chapters in this book are divided into four parts: Part One looks at the entire native antigen and covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography. Part Two talks about protein fragments derived from antigens, and discusses binding regions within antigen sequence using bacterial surface display and ELISA, for example. Part Three describes the use of surface plasmon resonance spectroscopy and biolayer interferometry, and Part Four highlights methods used to identify new antigens and assess antibody cross-reactivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Epitope Mapping Protocols, Third Edition is a valuable resource for anyone interested in furthering their research in this expanding field
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocol, viewed May 1, 2018)
Subject Antigenic determinants -- Research -- Methodology
Antigen-antibody reactions -- Research -- Methodology
Antigenic determinants.
Epitope Mapping -- methods
Medical -- Immunology.
Antigenic determinants
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Rockberg, Johan, editor
Nilvebrant, Johan, editor
ISBN 9781493978410