Title Methods in alcohol-related neuroscience research / edited by Yuan Liu, David M. Lovinger

Published Boca Raton : CRC Press, [2002]

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Description 1 online resource (391 pages, 4 unnumbered pages of plates) : illustrations (some color) Series Methods & new frontiers in neuroscience Methods & new frontiers in neuroscience series. Contents Ch. 1. Quantitative Trait Locus (QTL) Mapping in Mice / Abraham A. Palmer and Tamara J. Phillips -- Ch. 2. Knockout and Knockin Mice / Gregg E. Homanics -- App. 2.1. Preparation of Mouse Embryonic Fibroblast Feeder Cells -- App. 2.2. ES Cell Transformation by Electroporation -- Ch. 3. Molecular Studies by Chimeragenesis and Mutagenesis / Michael J. Beckstead, Gregory F. Lopreato and Kent E. Vrana / [and others] -- Ch. 4. Combining Patch-Clamp Recording and Gene Profiling in Single Neurons / Hermes H. Yeh, Stavros Therianos and Shao-Ming Lu -- App. 4.1. Protocol for Single-Cell mRNA Amplification/Reverse Northern Analysis -- App. 4.2. Protocol for Single-Cell RT-PCR -- App. 4.3. Protocol for Single-Cell Multiplex RT-PCR -- Ch. 5. Quantitative Analysis of Neurotransmitter Receptor Expression with Antibody-Based Techniques / C. Fernando Valenzuela and Daniel D. Savage -- App. 5.1. Detailed Protocols for Western Immunoblotting -- Ch. 6. Artificial Bilayer Techniques in Ion Channel Study / Steven N. Treistman, Robert O'Connell and John Crowley -- Ch. 7. Rapid Drug Superfusion and Kinetic Analysis / David M. Lovinger, Suzanne Sikes and Qing Zhou -- Ch. 8. Electrophysiological Assessment of Synaptic Transmission in Brain Slices / Jeff L. Weiner -- Ch. 9. Cognitive Correlates of Single Neuron Activity in Task-Performing Animals / Bennet Givens and T. Michael Gill -- Ch. 10. Multichannel Neural Ensemble Recording During Alcohol Self-Administration / Patricia H. Janak -- Ch. 11. Combined Single-Neuron Recording and Microdialysis in the Brain of Freely Behaving Animals (Rodents and Non-Human Primates) / Nandor Ludvig -- Ch. 12. Quantitative Microdialysis for in vivo Studies of Pharmacodynamics / Rueben A. Gonzales, Amanda Tang and Donita L. Robinson -- Ch. 13. Structural Magnetic Resonance Imaging (MRI) of the Human Brain / Terry L. Jernigan -- Ch. 14. Biochemical, Functional and Microstructural Magnetic Resonance Imaging (MRI) / Elfar Adalsteinsson, Edith V. Sullivan and Adolf Pfefferbaum Summary This text covers the methods, technologies, and approaches as applied to alcohol-related basic and clinical research. The neurophysiological basis of alcohol abuse and the methods used to study the potential causes, are discussed Bibliography Includes bibliographical references and index Subject Alcohol -- Physiological effect -- Research -- Methodology Brain -- Pathophysiology -- Research -- Methodology Alcoholism -- Molecular aspects -- Research -- Methodology Alcohol-Induced Disorders, Nervous System -- physiopathology Neurology -- methods Research Design HEALTH & FITNESS -- Diseases -- Nervous System (incl. Brain) MEDICAL -- Neurology. Form Electronic book Author Liu, Yuan, Ph. D. Lovinger, David M. (David Michael), 1959- ISBN 9781420042092 1420042092 9780429129117 0429129114

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