| Description |
1 online resource (streaming video file) (499 secs.) |
| Series |
Biology |
| Summary |
We describe a method to generate saturating transposon mutant libraries in Gram-negative bacteria and subsequent preparation of DNA amplicon libraries for high-throughput sequencing. As an example, we focus on the ESKAPE pathogen, Acinetobacter baumannii, but this protocol is amenable to a wide range of Gram-negative organisms |
| Audience |
For undergraduate, graduate, and professional students |
| Notes |
English |
|
Description based on online resource; title from resource description page |
| Subject |
Biology
|
| Form |
Streaming audio
|
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