| Description |
1 online resource (ix, 333 pages) : illustrations |
| Series |
Infectious disease (Totowa, N.J.)
|
| Contents |
Gene Expression Control At The Mrna Level -- Overview -- Messenger RNA Accumulation -- Transcript Profiling -- Transcriptional Regulation of Gene Expression -- Posttranscriptional Processing of Messenger RNA -- Regulation Of Protein Levels And Transcription Factor Function -- Overview -- Protein Synthesis and Turnover -- Proteomics -- Determination of Protein-Protein Interactions and the Motifs That Mediate Them -- Posttranslational Modifications -- Dissection of Signaling Pathways -- Use Of Transgenic And Knockout Mice To Study Gene Regulation -- Overview -- Production of Transgenic Animals -- Knockout Animals -- Isolation of Cells and Cell Lines From Transgenic and Knockout Animals |
| Summary |
Regulation of Gene Expression: Molecular Mechanisms presents a comprehensive overview of methods and approaches for characterizing mechanisms of gene regulation. The text is appropriate both as a graduate textbook and a standard laboratory reference and provides the essential groundwork for an advanced understanding of the various mechanisms that may result in altered activity of a specific cell protein. Each of three sections explores mechanisms of gene regulation and expression, and presents methods and protocols for achieving specific experimental goals. Part I focuses on approaches for studying control of mRNA expression and determining target genes for a given transcription copy. Part II outlines the methods for determining how proteins can regulate each other by mediating synthesis, degradation, protein-protein interactions, and posttranslational modification. Part III explores how gene targeting techniques in mice can provide insight into protein function. This volume provides a clear, concise overview of the protocols and techniques used to examine chemically or disease-mediated alterations in gene expression in mammalian systems |
| Bibliography |
Includes bibliographical references (pages 323-328) and index |
| Subject |
Genetic regulation.
|
|
Gene Expression Regulation -- physiology
|
|
Molecular Biology
|
|
Gene Expression Regulation
|
|
SCIENCE -- Life Sciences -- Genetics & Genomics.
|
|
Biomédecine.
|
|
Sciences de la vie.
|
|
Genetic regulation
|
| Form |
Electronic book
|
| Author |
Vanden Heuvel, John P.
|
|
Peters, Jeffrey M. (Jeffrey Maurice)
|
| LC no. |
2006020090 |
| ISBN |
1588292657 |
|
9781588292650 |
|
9781588292650 |
|
9781597452281 |
|
1597452289 |
|
128083269X |
|
9781280832697 |
|
