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Book Cover

Uniform Title Transgenic mouse (Larson)
Title Transgenic mouse : methods and protocols / edited by Melissa A. Larson
Published New York : Humana Press, [2020]


Description 1 online resource : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 2066
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 2066. 1064-3745
Springer protocols (Series) 1949-2448
Summary This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blastocyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed September 24, 2019)
Subject Transgenic mice.
Mice -- Genetic engineering
Mice as laboratory animals.
Mice, Transgenic -- genetics
Gene Transfer Techniques
Mice, Transgenic
Mice as laboratory animals
Mice -- Genetic engineering
Transgenic mice
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Larson, Melissa A., editor
ISBN 9781493998371