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Title Difference gel electrophoresis : methods and protocols / edited by Kay Ohlendieck
Published New York : Humana Press : Springer, ©2018


Description 1 online resource (x, 315 pages) : illustrations (some color)
Series Methods in molecular biology ; v. 1664
Methods in molecular biology (Clifton, N.J.) ; v. 1664. 1064-3745.
Contents Two-dimensional gel electrophoresis and 2D-DIGE / Paula Meleady -- Comparative DIGE proteomics / Kay Ohlendieck -- 2D-DIGE and fluorescence image analysis / Elisa Robotti and Emilio Marengo -- DIGE analysis software and protein identification approaches / Abduladim Hmmier and Paul Dowling -- Native DIGE : efficient tool to elucidate protein interactomes / Diksha Dani and Norbert A. Dencher -- Comparative two-dimensional fluorescence gel electrophoresis / Doreen Ackermann and Simone Konig -- DIGE-based phosphoproteomic analysis / Taras Stasyk and Lukas Alfons Huber -- DIGE saturation labeling for scarce amounts of protein from formalin-fixed paraffin-embedded (FFPE) tissue / Paul Dowling -- Comparative 3-sample DIGE analysis of skeletal muscles / Kay Ohlendieck -- DIGE analysis of ProteoMiner fractionated serum/plasma samples / Sandra Murphy and Paul Dowling -- DIGE analysis of human tissues / Cecilia Gelfi and Daniele Capitanio -- DIGE analysis of animal tissues / Alessio Di Luca, Ruth Hamill, Anne Maria Mullen, and Giuliano Elia -- Rapid 2D DIGE proteomic analysis of mouse liver / Shotaro Kamata and Isao Ishii -- Proteomic analysis of lung tissue by DIGE / Jarlath E. Nally and Simone Schuller -- Comparative testis tissue proteomics using 2-dye versus 3-dye DIGE analysis / Ashling Holland -- DIGE analysis of fish tissues / Joanna Nynca, Mariola A. Dietrich, and Andrzej Ciereszko -- Protein digestion for DIGE analysis / Sandra Murphy and Kay Ohlendieck -- Subcellular fractionation for DIGE-based proteomics / Sandra Murphy -- DIGE analysis of immunodepleted plasma / Paul Dowling and Kay Ohlendieck -- Elucidating cellular metabolism and protein difference data from DIGE proteomics experiments using enzyme assays / Andrew Dowd -- Enzyme assay methods to validate DIGE proteomics data / Andrew Dowd -- Immunoblot analysis of DIGE-based proteomics / Martin Landsberger and Heinrich Brinkmeier -- Immunofluorescence microscopy for DIGE-based proteomics / Rustam R. Mundegar, Margit Zweyer, and Dieter Swandulla
Bibliography Includes bibliographical references and index
Subject Gel electrophoresis -- Laboratory manuals
Two-dimensional electrophoresis.
Polyacrylamide gel electrophoresis.
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Two-Dimensional Difference Gel Electrophoresis
Two-dimensional electrophoresis
Polyacrylamide gel electrophoresis
Gel electrophoresis
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Ohlendieck, Kay, editor
ISBN 9781493972685