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Title Th9 cells : methods and protocols / edited by Ritobrata Goswami
Published New York, NY : Humana Press : Springer, 2017


Description 1 online resource (x, 259 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 1585
Methods in molecular biology (Clifton, N.J.) ; v. 1585. 1064-3745
Contents Th9 cells : new member of T helper cell family / Ritobrata Goswami -- IL-9 : function, sources, and detection / Wilmer Gerardo Rojas-Zuleta and Elizabeth Sanchez -- IL-9 signaling pathway : an update / Dijendra Nath Roy and Ritobrata Goswami -- Method to in vitro differentiate Th9 cells from mouse naïve CD4+ T cells / Duy Pham -- T cell receptor and co-stimulatory signals for Th9 generation / Françoise Meylan and Julio Gomez-Rodriguez -- Polarizing cytokines for human Th9 cell differentiation / Prabhakar Putheti -- Determining the frequencies of Th9 cells from whole blood / Anuradha Rajamanickam and Subash Babu -- IL-9 production by nonconventional T helper cells / Silvia C.P. Almeida and Luis Graca -- Prediction and validation of transcription factors binding sites in the Il9 locus / William Orent and Wassim Elyaman -- Flow cytometric assessment of STAT molecules in Th9 cells / Lucien P. Garo, Vanessa Beynon, and Gopal Murugaiyan -- Transcription factors downstream of IL-4 and TGF-[beta] signals : analysis by quantitative PCR, western blot, and flow cytometry / Atsushi Sugimoto, Ryoji Kawakami, and Norihisa Mikami -- Retroviral transduction and reporter assay : transcription factor cooperation in Th9 cell development / Rukhsana Jabeen -- Transcription factor binding studies in CD4+ T cells : siRNA transfection, chromatin immunoprecipitation, and liquid luminescent DNA precipitation assay / Etienne Humblin, François Ghiringhelli, and Frédérique Végran -- Defining epigenetic regulation of the interleukin-9 gene by chromatin immunoprecipitation / Alla Skapenko and Hendrik Schulze-Koops -- Allergic inflammation and atopic disease : role of Th9 cells / Pornpimon Angkasekwinai -- Characterization of Th9 cells in the development of EAE and IBD / Sakshi Malik, Valerie Dardalhon, and Amit Awasthi -- B16 lung melanoma model to study the role of Th9 cells in cancer / Alka Dwivedi, Sushant Kumar, and Rahul Purwar -- Th9 cells and parasitic inflammation : use of Nippostrongylus and Schistosoma models / Miguel Enrique Serrano Pinto and Paula Licona-Limón -- Isolation and purification of Th9 cells for the study of inflammatory diseases in research and clinical settings / Patricia Keating and James X. Hartmann
Summary This volume presents comprehensive laboratory protocols that have been used to generate Th9 cells, both in vitro and in vivo. The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and comprehensive, Th9 Cells: Methods and Protocols is a valuable resource for scientists in this field and will provide them with techniques to generate Th9 cells for specific downstream events
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocol, viewed May 8, 2017)
Subject T cells -- Laboratory manuals
T cells.
T-Lymphocytes, Helper-Inducer
CD4-Positive T-Lymphocytes
Cellular biology (cytology)
Science -- Life Sciences -- Biology -- Molecular Biology.
T cells
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Goswami, Ritobrata, editor
ISBN 9781493968770