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Title CD95 : methods and protocols / edited by Patrick Legembre
Published New York : Humana Press : Springer, ©2017


Description 1 online resource (xi, 230 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; 1557
Methods in molecular biology (Clifton, N.J.) ; v. 1557. 1064-3745
Contents Production of the non-apoptotic metalloprotease-cleaved CD95L and its cytotoxic recombinant counterpart designed Ig-CD95L / Eléonore Lepvrier, Sophie Martin, and Brigitte Collet -- CD95 stimulation with CD95L and DISC analysis / Matthieu Le Gallo and Patrick Legembre -- Immunoprecipitation of death inducing signaling complex by caspase-8 / Andrei Alexandru Constentinescu, Aymeric Morlé, and Olivier Micheau -- In vitro evaluation of the apoptosis function in human activated T cells / Aude Magerus-Chatinet and Frédéric Rieux-Laucat -- Proximity ligation assay (PLA) to evaluate DISC and MISC composition / Melissa Thomas and Patrick Legembre -- Fluorometric methods for detection of mitochondrial membrane depolarization induced by CD95 activation / Kenji F. Shoji and Laure Debure -- Generation and application of bioluminescent CD95 ligand fusion proteins / Isabell Lang, Juliane Kums, and Harald Wajant -- CD95-mediated calcium signaling / Mehdi Hammadi, Vanessa Delcroix, Anne-Marie Vacher, Thomas Ducret, and Pierre Vacher -- CD95-mediated proton regulation / Auréa Cophignon, Mallorie Poët, Michael Monet, Michel Tauc, and Laurent Counillon -- Study of the CD95-mediated non-apoptotic signaling pathway : PI3K / Amélie Fouqué and Patrick Legembre -- Organelle separation and cell signaling / Leandro Silva Da Costa and Damien Arnoult -- Boyden chamber assay to study of cell migration induced by metalloprotease cleaved-CD95L / Amanda Poissonnier and Patrick Legembre -- Isolation of lipid rafts through discontinuous sucrose gradient centrifugation and Fas/CD95 death receptor localization in raft fractions / Consuelo Gajate and Faustino Mollinedo -- Quantifying CD95/cl-CD95L implications in cell mechanics and membrane tension by atomic force microscopy based force measurements / Anaïs Sadoun and Pierre-Henri Puech -- Sketching of CD95 oligomers by in silico investigations / Nicolas Levoin -- Site-specific detection of tyrosine phosphorylated CD95 following protein separation by conventional and phospho-protein affinity SDS-PAGE / Krittalak Chakrabandhu, Sébastien Huault, and Anne-Odile Hueber -- Detection of S-acylated CD95 by acyl-biotin exchange / Aurelie Rossin and Anne-Odile Hueber -- Exploration of Fas S-nitrosylation by the biotin switch assay / Ali Bettaieb, Catherine Paul, and Stéphanie Plenchette -- Method to measure sphingomyelin synthase activity changes in response to CD95L / Fatima Bilal, Michaël Pérès, Nathalie Andrieu-Abadie, Thierry Levade, Bassam Badran, Ahmad Daher, and Bruno Ségui -- Liquid chromatography-high resolution mass spectrometry method to study sphingolipid metabolism changes in response to CD95L / Fatima Bilal, Michaël Pérès, Pauline Le Faouder, Aude Dupuy, Justine Bertrand-Michel, Nathalie Andrieu-Abadie, Thierry Levade, Bassam Badran, Ahmad Daher, and Bruno Ségui -- CD95 and the MRL-lpr mouse model / Robin J. Flynn
Summary This volume provides the most commonly used methods and protocols to study the apoptotic and non-apoptotic roles of CD95. Chapters explore molecular, biochemical, cellular methods and animal models to in order to better understand the biological functions of this cytokine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, CD95: Methods and Protocols aims to foster original research on CD95/CD95L couple
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocol, viewed January 18, 2017)
Subject Cysteine proteinases -- Laboratory manuals
Apoptosis -- Laboratory manuals
Tumor necrosis factor -- Receptors -- Laboratory manuals
Cellular signal transduction.
fas Receptor -- physiology
Fas Ligand Protein -- physiology
Signal Transduction
Science -- Life Sciences -- Biochemistry.
Cellular signal transduction
Cysteine proteinases
Tumor necrosis factor -- Receptors
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Legembre, Patrick, editor
ISBN 9781493967803
Other Titles CD 95