| Description |
1 online resource (xv, 353 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1422 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1422. 1064-3745
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| Contents |
CRISPR/Cas9-mediated genome editing of mouse small intestinal organoids / Gerald Schwank and Hans Clevers -- Lentivirus-based stable gene delivery into intestinal organoids / Yoshiaki Maru, Kaoru Orihashi, and Yoshitaka Hippo -- Co-culture of gastric organoids and immortalized stomach mesenchymal cells / Nina Bertaux-Skeirik [and others] -- Air-liquid interface culture system for 3D organoid culture of diverse primary gastrointestinal tissues / Xingnan Li, Akifumi Ootani, and Calvin Kuo -- Organotypical tissue cultures from fetal and neonatal murine colon / Peter H. Neckel and Lothar Just -- Ussing chamber technique to measure intestinal epithelial permeability / Sadasivan Vidyasagar and Gordon MacGregor -- HPLC-based metabolomic analysis of normal and inflamed gut / Daniel J. Kao [and others] -- NMR-based metabolomic analysis of normal and inflamed gut / Daniel J. Kao [and others] -- Detecting reactive oxygen species generation and stem cell proliferation in the Drosophila intestine / Liping Luo, April R. Reedy, and Rheinallt M. Jones -- Imaging Inflammatory hypoxia in the murine gut / Alyssa K. Whitney and Eric L. Campbell -- Label-free imaging of eosinophilic esophagitis mouse models using optical coherence tomography / Aneesh Alex [and others] -- Near-infrared fluorescence endoscopy to detect dysplastic lesions in the mouse colon / Elias Gounaris [and others] -- Visualization of signaling molecules during neutrophil recruitment in transgenic mice expressing FRET biosensors / Rei Mizuno [and others] -- In vivo myeloperoxidase imaging and flow cytometry analysis of intestinal myeloid cells / Jan Hülsdünker and Robert Zeiser -- Macrophage isolation from the mouse small and large intestine / Akihito Harusato, Duke Geem, and Timothy L. Denning -- Isolation and functional analysis of lamina propria dendritic cells from the mouse small intestine / Naoki Takemura and Satoshi Uematsu -- Purification and adoptive transfer of group 3 gut innate lymphoid cells / Xiaohuan Guo [and others] -- Immunotherapy with iTreg and nTreg cells in a murine model of inflammatory bowel disease / Dipica Haribhai, Talal A. Chatila, and Calvin B. Williams -- Isolation of eosinophils from the lamina propria of the murine small intestine / Claudia Berek, Alexander Beller, and Van Trung Chu -- Investigation of host and pathogen contributions to infectious colitis using the Citrobacter rodentium mouse model of infection / Else S. Bosman [and others] -- Murine trinitrobenzoic acid-induced colitis as a model of crohn's disease / John F. Kuemmerle -- Oxazolone-induced colitis as a model of Th2 immune responses in the intestinal mucosa / Benno Weigmann and Markus F. Neurath -- Mongolian gerbil : a robust model of Helicobacter pylori-induced gastric inflammation and cancer / Jennifer M. Noto [and others] -- Rapid screenable assay for compounds that protect against intestinal injury in zebrafish larva / Jason R. Goldsmith, Sarah Tomkovich, and Christian Jobin -- AOM/DSS model of colitis-associated cancer / Bobak Parang, Caitlyn W. Barrett, and Christopher S. Williams -- Characterization of colorectal cancer development in apcmin/+ mice / ILKe Nalbantoglu, Valerie Blanc, and Nicholas O. Davidson -- Modeling murine gastric metaplasia through tamoxifen-induced acute parietal cell loss / Jose B. Saenz, Joseph Burclaff, and Jason C. Mills -- Hamster buccal pouch model of oral carcinogenesis / Siddavaram Nagini and Jaganathan Kowshik |
| Summary |
This volume provides a comprehensive collection of classical and cutting edge protocols and techniques to examine the normal development and physiological functions of the gastrointestinal system and to model the most common digestive diseases. The chapters focus on diverse research topics including ex vivo systems to study gastrointestinal development and functions, in vivo imaging of the gastrointestinal tract, isolation and characterization of intestinal immune cells, and animal models of gastrointestinal inflammation and cancer. The Gastrointestinal Physiology and Diseases: Methods and Protocols book targets wide audience of physiologists, cell and developmental biologists, immunologists, and physician-scientists working in the field of gastroenterology and beyond. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Highly practical and clearly written, Gastrointestinal Physiology and Diseases: Methods and Protocols will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining normal functions and diseases of the gastrointestinal tract |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (springerLink, viewed June 2, 2016) |
| Subject |
Gastrointestinal system -- Pathophysiology
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Diseases -- Animal models.
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Gastrointestinal Tract -- physiology
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Gastrointestinal Diseases -- physiopathology
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Disease Models, Animal
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Medical research.
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Diseases -- Animal models
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Gastrointestinal system -- Pathophysiology
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Ivanov, Andrei I., editor.
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| ISBN |
9781493936038 |
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1493936034 |
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1493936018 |
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9781493936014 |
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