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Title Cytokine bioassays : methods and protocols / edited by Ivana Vancurova
Published New York, NY : Humana Press, 2014


Description 1 online resource (xiv, 367 pages) : illustrations (some color)
Series Methods in Molecular Biology, 1940-6029 ; 1172
Methods in molecular biology (Clifton, N.J.) ; v. 1172. 1064-3745
Contents Control of pro-inflammatory cytokine release from human monocytes with the use of an interleukin-10 monoclonal antibody / Hardik Patel and Dennis Davidson -- Cytokine-induced neutrophil chemotaxis assay / Dennis Davidson and Hardik Patel -- In vitro one-dimensional assay to study growth factor-regulated tumor cell-macrophage interaction / Ved P. Sharma, Brian T. Beaty, Dianne Cox, John S. Condeelis, and Robert J. Eddy -- Chemotactic responses by macrophages to a directional source of a cytokine delivered by a micropipette / Michael Cammer and Dianne Cox -- Assessment of phagocytic activity of cultured macrophages using fluorescence microscopy and flow cytometry / Lokesh Sharma ... [e tal.] -- Assessment of cytokine-modulated proteasome activity / Christopher J. Kirk, Saul R. Powell, and Edmund J. Miller
Evaluation of the adverse effect of low concentration of cadmium on interleukin-4 induced class switch recombination in burkett's lymphoma Raji cell line / Vladimir Poltoratsky -- Mix-and-measure assay for determining the activation status of endogenous Cdc42 in cytokine-stimulated macrophage cell lysates / Veronika Miskolci [and others] -- Analysis of the cell surface expression of cytokine receptors using the surface protein biotinylation method / Mahmud Arif Pavel -- Detection of CXCR2 cytokine receptor surface expression using immunofluorescence / Clarissa Lam [and others] -- Detecting Tie2, an endothelial growth factor receptor, by using immunohistochemistry in mouse lungs / Prajna P. Guha, Sascha A. David, and Chandra C. Ghosh -- Use of shRNA for stable suppression of chemokine receptor expression and function in human cancer cell lines / Nicole Salazar [and others]
Intracellular staining and detection of cytokines by fluorescence-activated flow cytometry / Giulia Freer -- Cytokine detection by flow cytometry / Jian-Ge Qiu [and others] -- Analysis of IL-17 production by flow cytometry and ELISPOT assays / Ling Zhao [and others] -- Interleukin-1 (IL-1) immunohistochemistry assay in oral squamous cell carcinoma / Takaaki Kamatani -- Immunofluorescence and subsequent confocal microscopy of intracellular TNF in human neutrophils / Janet Rollins and Veronika Miskolci -- Evaluating cytoplasmic and nuclear levels of inflammatory cytokines in cancer cells by western blotting / Himavanth R. Gatla [and others] -- Western analysis of intracellular interleukin-8 in human mononuclear leukocytes / Veronika Miskolci [and others] -- Quantitative analysis of bortezomib-induced IL-8 gene expression in ovarian cancer cells / Bipradeb Singha [and others]
Summary Cytokine Bioassays: Methods and Protocols provides a comprehensive collection of classic and cutting-edge methodologies that are used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. Chapters are divided into three main categories, the first category details the immunodetection of released cytokines in tissue culture supernatants, plasma, serum, and whole blood samples by immunoassays. The second part focuses on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced phagocytosis assay, proteasome activity assay, and analysis of cytokine-induced immunoglobulin class switching. Part three presents analysis of intracellular cytokines by flow cytometry, immunohistochemistry, immunofluorescence confocal microscopy, and western blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cytokine Bioassays: Methods and Protocols is written by experienced, basic, and clinical researchers with hands-on knowledge of the described protocols. This book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research
Analysis biomedische wetenschappen
growth factors
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed June 18, 2014)
Subject Cytokines -- Laboratory manuals
Biological assay -- Laboratory manuals
Biological assay.
Biological Assay
Biosensing Techniques
Biological assay
Genre/Form Laboratory manuals
Form Electronic book
Author Vancurova, Ivana, editor.
ISBN 9781493909285