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Title Cytochrome P450 protocols / edited by Ian R. Phillips and Elizabeth A. Shephard
Published Totowa, N.J. : Humana Press, ©1998


Description 1 online resource (xiv, 482 pages) : illustrations
Series Methods in molecular biology ; v. 107
Methods in molecular biology (Clifton, N.J.) ; volume 107
Contents The role of methods in the discovery of the cytochromes P450 / R.W. Estabrook -- Cytochrome P450 nomenclature / D.R. Nelson -- Spectral analyses of cytochromes P450 / J.B. Schenkman and I. Jansson -- Purification of cytochromes P450. Rat and human hepatic forms / F.P. Guengerich and M.V. Martin -- Isolation and purification of constitutive forms of microsomal cytochrome P450 / J.B. Schenkman and I. Jansson -- Purification of extrahepatic cytochromes P450 / M. Warner and J.A. Gustafsson -- Purification of cytochromes P450: Products of bacterial recombinant expression systems / F.P. Guengerich, N.A. Hosea and M.V. Martin -- Cytochrome P450 reconstitution systems / T. Shimada and H. Yamazaki -- Catalytic assays for human cytochrome P450 / T.K. Chang and D.J. Waxman -- Enzymatic analysis of cdna-expressed human CYP1a1, CYP1A2, and CYP1B1 with 7-ethoxyresorufin as substrate / T.K. Chang and D.J. Waxman -- Spectrofluorometric analysis of CYP2A6-catalyzed coumarin 7-hydroxylation / D.J. Waxman and T.K. Chang -- Determination of the CYP2B6 component of 7-ethoxy-4-trifluoromethylcoumarin o-deethylation activity in human liver microsomes / T.K. Chang, C.L. Crespi and D.J. Waxman -- High-performance liquid chromatographic analysis of CYP2C8-catalyzed paclitaxel 6 alpha-hydroxylation / C.L. Crespi, T.K. Chang and D.J. Waxman -- Determination of CYP2C9-catalyzed diclofenac 4'-hydroxylation by high-performance liquid chromatography / C.L. Crespi, T.K. Chang and D.J. Waxman -- CYP2C19-mediated (s)-mephenytoin 4'-hydroxylation assayed by high-performance liquid chromatography with radiometric detection / C.L. Crespi, T.K. Chang and D.J. Waxman -- CYP2D6-dependent bufuralol 1'-hydroxylation assayed by reversed-phase ion-pair high-performance liquid chromatography with fluorescence detection / C.L. Crespi, T.K. Chang and D.J. Waxman -- Spectrophotometric analysis of human CYP2E1-catalyzed p-nitrophenol hydroxylation / T.K. Chang, C.L. Crespi and D.J. Waxman -- Thin-layer chromatographic analysis of human CYP3A-catalyzed testosterone 6 beta-hydroxylation / D.J. Waxman and T.K. Chang -- Determination of CYP4A11-catalyzed lauric acid 12-hydroxylation by high-performance liquid chromatography with radiometric detection / C.L. Crespi, T.K. Chang and D.J. Waxman -- An isocratic high-performance liquid chromatographic assay for CYP7A1-catalyzed cholesterol 7 alpha-hydroxylation / D.J. Waxman and T.K. Chang -- Use of 7-ethoxycoumarin to monitor multiple enzymes in the human CYP1, CYP2, and CYP3 families / D.J. Waxman and T.K. Chang -- Expression of eukaryotic cytochromes P450 in e. Coli / C.M. Jenkins, I. Pikuleva and M.R. Waterman -- Expression of mammalian cytochromes P450 in yeast / Y. Yabusaki -- Expression of cytochromes P450 in a baculovirus system / S.R. Hood, G. Shah and P. Jones -- Expression of cytochromes P450 in mammalian cells / E.M. de Groene -- Monoclonal antibodies to cytochromes P450 / H.V. Gelboin [and others] -- Targeting antipeptide antibodies towards cytochrome P450 enzymes / R.J. Edwards -- Signals for retention of cytochrome P450 in the endoplasmic reticulum / E. Skorupa and B. Kemper -- Quantification of cytochrome P450 gene expression by rnase protection analysis / C.N. Palmer -- Hepatocyte cultures in drug metabolism and toxicological research and testing / V. Rogiers and A. Vercruysse -- Isolation of rat hepatocytes / K. De Smet [and others] -- Collagen-gel cultures of rat hepatocytes: Collagen-gel sandwich and immobilization cultures / S. Beken [and others] -- Rat hepatocyte cultures. Conventional monolayer cultures and cocultures with rat liver epithelial cells / T. Vanhaecke [and others] -- Isolation of periportal and pericentral hepatocytes / R. Gebhardt -- Perifusion culture of hepatocytes / R. Gebhardt -- Human hepatocyte culture / J.B. Ferrini [and others] -- Reconstruction of human epidermis in vitro / M.C. Lenoir-Viale -- Gene transfer and expression / F. Gaunitz and M. Papke -- Lipid-mediated gene transfer into normal adult human hepatocytes in primary culture / J.C. Ourlin [and others] -- Identification of regulatory protein-binding sites in cytochrome P450 genes by means of the gel-retardation assay / H. Dell [and others] -- Cytochrome P450 gene regulation. Analysis of protein-DNA interactions in situ / S.T. Okino and J.P. Whitlock, Jr. -- Analysis of cytochrome P450 polymorphisms / A.K. Daly [and others] -- Induction of cytochrome P450 1A (CYP1A) in fish. A biomarker for environmental pollution / B.M. Nilsen, K. Berg and A. Goksoyr -- Generation of allelic series in mice by gene targeting / M.K. Bunger and C.A. Bradfield
Summary In Cytochrome P450 Protocols, Ian Phillips and Elizabeth Shephard assemble a comprehensive collection of cutting-edge techniques for the investigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of antibodies, as well as methods for quantification of gene expression, transfection of hepatocytes, and for the investigation of DNA-protein interactions and genetic polymorphisms. In addition, because of the growing importance of in vitro systems in pharmacological toxicology, the book contains techniques for the culture of rodent and human hepatocytes and human epidermis. Cytochrome P450 induction as a biomarker for environmental pollution and the generation of mice with targeted gene disruptions complete this exhaustive collection of core techniques. Cytochrome P450 Protocols includes in one volume both state-of-the-art and classic methods that have not been superseded but remain extremely useful. The collection provides both novice and experienced researchers across many fields-toxicology, pharmacology, environmental biology, biochemistry, and molecular biology-all the tools needed to elucidate the crucial biological role played by cytochromes P450 in the metabolism of therapeutic drugs, chemical carcinogens, and environmental pollutants
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. MiAaHDL
Print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
Subject Cytochrome P-450 -- Laboratory manuals
Cytochrome P-450.
Cytochrome P-450 Enzyme System
Cytochrome P-450.
Cytochroom P450.
Wetenschappelijke technieken.
Genre/Form Laboratory manuals.
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Phillips, Ian R. (Ian Randall), 1951-
Shephard, Elizabeth A. (Elizabeth Anne), 1950-
ISBN 9781592595808
Other Titles Cytochrome P four hundred fifty protocols