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Title Mycobacteria protocols / edited by Tanya Parish and N.G. Stoker
Published Totowa, N.J. : Humana Press, [1998]


Location Call no. Vol. Availability
 W'PONDS  579.374 Par/Mpr  AVAILABLE
Description xiv, 472 pages : illustrations ; 24 cm
Series Methods in molecular biology ; 101
Methods in molecular biology (Clifton, N.J.) ; v. 101
Contents Machine derived contents note: Mycobacteria: Bugs and Bugbears -- Tanya Parish and Neil G. Stoker -- Mycobacteria: General Culture Methodology and Safety Considerations -- Bryan W. Allen -- Isolation of Genomic DNA from Mycobacteria -- John T. Belisle and Michael G. Sonnenberg -- Nucleic-Acid Extraction: Plasmid/Cosmid DNA -- Bonnie B. Plikaytis and C. Harold King -- Pulsed Field Gel Electrophoresis for Mycobacteria -- Wolfgang J. Philipp,Stephen Gordon, Amalio Telenti, and Stewart T. Cole -- Extraction of RNA from Mycobacteria -- Eshwar Mahenthiralingam -- Preparation of Cell-Free Extracts from Mycobacteria -- Tanya Parish and Paul R. Wheeler -- Preparation of Cell-Wall Fractions from Mycobacteria -- Gurdyal S. Besra -- Using MycDB on the World-Wide Web -- Staffan Bergh and Stewart T. Cole -- Conjugating DNA into Mycobacteria -- Julie-Ann Gavigan and Carlos Martin -- Electroporation of Mycobacteria -- Tanya Parish and Neil G. Stoker -- Mycobacteriophages -- Gary J. Sarkis and Graham F. Hatfull -- Chemical Mutagenesis of Mycobacteria -- Lucy A. Brooks -- Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System -- Esther Přez, Julie-Ann Gavigan, Isabel Otal, Christophe Guilhot, Vladimir Pelicic, Brigitte Giguel, and Carlos Martin -- Homologous Recombination in Mycobacterium smegmatis: Screening Methods for Detection of Gene Replacement -- Robert N. Husson -- Gene Replacement in Mycobacterium smegmatis Using a Dominant Negative Selectable Marker -- Peter Sander and Erik C. Bṯtger -- Gene Replacement in Mycobacterium intracellulare -- Britt-Inger Marklund and Richard W. Stokes -- Gene Replacement in Mycobacterium bovis BCG -- Elizabeth Norman -- The Application of Bacterial Luciferase as a Reporter Gene in Mycobacteria -- Stephen Gordon, Gavin Chung, and Peter Andrew -- Green Fluorescent Protein: A Tool for Gene Expression and Cell Biology in Mycobacteria -- Laura E. Via, Subramanian Dhandayuthapani, Dusanka Deretic, and V. Deretic -- Expression of Genes in Mycobacteria -- Peadar - Gaora -- Secretion of Mammalian Proteins from Mycobacteria -- Peter J. Murray and Richard A. Young -- Intracellular Gene Expression: Analysis of RNA from Mycobacteria in Macrophages Using RT-PCR -- Philip D. Butcher, Joseph A. Mangan, and Irene M. Monahan -- Analysis of Mycobacterial Differential Gene Expression by RAP-PCR -- Joseph A. Mangan and Philip D. Butcher -- Mycobacterial Speciation -- Francis A. Drobniewski and Anne H. C. Uttley -- Species Identification of Mycobacteria Using rDNA Sequencing -- Philip Kirschner and Erik C. Bṯtger -- Detection of Mycobacterium tuberculosis by a Colorimetric Polymerase Chain Reaction -- Stuart M. Wilson -- Rapid Detection and Simultaneous Strain Differentiation of Mycobacterium tuberculosis Complex Bacteria by Spoligotyping -- Henri O. F. Molhuizen, Annelies E. Bunschoten, Leo M. Schouls, and Jan D. A. van Embden -- Computer Analysis of IS6110 RFLP Patterns of Mycobacterium tuberculosis -- Herre F. Heersma, Kristin Kremer, and Jan D. A. van Embden -- Detection of Mutations in Mycobacteria by PCR-SSCP(Single-Strand Conformation Polymorphism) -- Amalio Telenti, Nadine Honor,̌ and Stewart T. Cole -- The Use of Luciferase-Reporter Phage for Antibody-Specific Testing of Mycobacteria -- Paul F. Riska and William R. Jacobs, Jr -- Index
Notes Formerly CIP. Uk
Bibliography Includes bibliographical references and index
Notes Also available online via the World Wide Web, by subscription to (BMP) via Humana Press
Subject Bacterial genetics -- Laboratory manuals.
Mycobacteria -- Laboratory manuals.
Mycobacterium -- genetics.
DNA -- physiology.
Molecular Biology -- methods.
Mycobacterium -- growth & development.
DNA -- physiology.
Molecular Biology -- methods.
Mycobacterium -- genetics.
Mycobacterium -- growth & development.
Author Parish, Tanya.
Stoker, Neil G.
Humana Press.
LC no. 98022177
ISBN 0896034712 (alk. paper)